Evaluating noninvasive methods of sex identification in Green Sea Turtle (Chelonia mydas) hatchlings
Document Type
Journal article
Source Publication
Chelonian Conservation and Biology
Publication Date
7-2011
Volume
10
Issue
1
First Page
117
Last Page
123
Publisher
Chelonian Research Foundation
Keywords
temperature sex determination, Estradiol, Testosterone, Guangdong Province, Sea Turtle Bay
Abstract
The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle (Chelonia mydas) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.
DOI
10.2744/CCB-0852.1
Print ISSN
10718443
E-ISSN
19433956
Publisher Statement
Copyright © 2011 Institute for Scientific Information. Access to external full text or publisher's version may require subscription.
Full-text Version
Publisher’s Version
Language
English
Recommended Citation
Xia, Z.-R., Li, P.-P., Gu, H.-X., Fong, J.J. & Zhao, E.-M. (2011). Evaluating noninvasive methods of sex identification in Green Sea Turtle (Chelonia mydas) hatchlings. Chelonian Conservation and Biology, 10(1), 117-123. doi: 10.2744/CCB-0852.1