Diversity and saline resistance of endophytic fungi associated with pinus thunbergii in coastal shelterbelts of Korea
Document Type
Journal article
Source Publication
Journal of Microbiology and Biotechnology
Publication Date
3-28-2014
Volume
24
Issue
3
First Page
324
Last Page
333
Publisher
Han'gug Mi'saengmul Saengmyeong Gong Haghoe,Korean Society for Microbiology and Biotechnology
Keywords
Coastal pine forest, endophyte community, salinity tolerance, shelterbelt
Abstract
The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.
DOI
10.4014/jmb.1310.10041
Print ISSN
10177825
E-ISSN
17388872
Publisher Statement
Copyright © 2014 by The Korean Society for Microbiology and Biotechnology. Access to external full text or publisher's version may require subscription.
Full-text Version
Publisher’s Version
Language
English
Recommended Citation
Min, Y. J., Park, M. S., Fong, J. J., Quan, Y., Jung, S., & Lim, Y. W. (2014). Diversity and saline resistance of endophytic fungi associated with pinus thunbergii in coastal shelterbelts of Korea. Journal of Microbiology and Biotechnology, 24(3), 324–333. doi: 10.4014/jmb.1310.10041