Sequence validation for the identification of the white-rot fungi Bjerkandera in public sequence databases
Document Type
Journal article
Source Publication
Journal of Microbiology and Biotechnology
Publication Date
10-2014
Volume
24
Issue
10
First Page
1313
Last Page
1319
Publisher
Han'gug Mi'saengmul Saengmyeong Gong Haghoe,Korean Society for Microbiology and Biotechnology
Keywords
Sequence validation, ITS, 28S nuclear ribosomal large subunit, white-rot fungi, Bjerkandera, GenBank
Abstract
White-rot fungi of the genus Bjerkandera are cosmopolitan and have shown potential for industrial application and bioremediation. When distinguishing morphological characters are no longer present (e.g., cultures or dried specimen fragments), characterizing true sequences of Bjerkandera is crucial for accurate identification and application of the species. To build a framework for molecular identification of Bjerkandera, we carefully identified specimens of B. adusta and B. fumosa from Korea based on morphological characters, followed by sequencing the internal transcribed spacer region and 28S nuclear ribosomal large subunit. The phylogenetic analysis of Korean Bjerkandera specimens showed clear genetic differentiation between the two species. Using this phylogeny as a framework, we examined the identification accuracy of sequences available in GenBank. Analyses revealed that many Bjerkandera sequences in the database are either misidentified or unidentified. This study provides robust reference sequences for sequence-based identification of Bjerkandera, and further demonstrates the presence and dangers of incorrect sequences in GenBank.
DOI
10.4014/jmb.1404.04021
Print ISSN
10177825
E-ISSN
17388872
Publisher Statement
Copyright© 2014 by The Korean Society for Microbiology and Biotechnology. Access to external full text or publisher's version may require subscription.
Full-text Version
Publisher’s Version
Language
English
Recommended Citation
Jung. P. E., Fong, J. J., Park, M. S., Oh, S.-Y., Kim, C., & Lim, Y. W. (2014). Sequence validation for the identification of the white-rot fungi Bjerkandera in public sequence databases. Journal of Microbiology and Biotechnology, 24(10), 1313-1319. doi: 10.4014/jmb.1404.04021