Title

Evaluating noninvasive methods of sex identification in Green Sea Turtle (Chelonia mydas) hatchlings

Document Type

Journal article

Source Publication

Chelonian Conservation and Biology

Publication Date

7-2011

Volume

10

Issue

1

First Page

117

Last Page

123

Publisher

Chelonian Research Foundation

Keywords

temperature sex determination, Estradiol, Testosterone, Guangdong Province, Sea Turtle Bay

Abstract

The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle (Chelonia mydas) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.

DOI

10.2744/CCB-0852.1

Print ISSN

10718443

E-ISSN

19433956

Publisher Statement

Copyright © 2011 Institute for Scientific Information. Access to external full text or publisher's version may require subscription.

Full-text Version

Publisher’s Version

Recommended Citation

Xia, Z.-R., Li, P.-P., Gu, H.-X., Fong, J.J. & Zhao, E.-M. (2011). Evaluating noninvasive methods of sex identification in Green Sea Turtle (Chelonia mydas) hatchlings. Chelonian Conservation and Biology, 10(1), 117-123. doi: 10.2744/CCB-0852.1