Delimitation of Russula subgenus Amoenula in Korea using three molecular markers
Hangug Gynnhaghoi,Korean Society of Mycology
DNA barcoding, Internal transcribed spacer, RNA polymerase II gene, Russula mariae, Russula violeipes, 28S nuclear ribosomal large subunit
Distinguishing individual Russula species has been difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. Due to highly similar macroscopic features, such as the presence of a red-cap, species identification within the Russula subgenus Amoenula is particularly difficult. Three species of the subgenus Amoneula have been reported in Korea. We used a combination of morphology and three molecular markers, the internal transcribed spacer (ITS), 28S nuclear ribosomal large subunit (LSU), and RNA polymerase II gene (RPB2), for identification and study of the genetic diversity of Russula subgenus Amoenula in Korea. We identified only two species in Korea (R. mariae and R. violeipes); these two species were indistinguishable according to morphology and LSU, but were found to be reciprocally monophyletic species using ITS and RPB2. The markers, ITS, LSU, and RPB2, have been tested in the past for use as DNA barcoding markers, and findings of our study suggest that ITS and RPB2 had the best performance for the Russulasubgenus Amoneula.
Copyright © The Korean Society of Mycology 2013. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Park, M. S., Fong, J. J., Lee, H., Oh, S.-Y., Jung, P. E., Min, Y. J, ... Lim, Y. W. (2013). Delimitation of Russula subgenus Amoenula in Korea using three molecular markers. Mycobiology, 41(4), 191-201. doi: 10.5941/MYCO.2013.41.4.191