Title

Marine-derived Penicillium in Korea : diversity, enzyme activity, and antifungal properties

Document Type

Journal article

Source Publication

Antonie van Leeuwenhoek

Publication Date

8-2014

Volume

106

Issue

2

First Page

331

Last Page

345

Publisher

Springer Netherlands

Keywords

Marine-derived fungi, Penicillium, Multi-gene phylogenetic approach, Alginase, β-Glucosidase, Antifungal activity

Abstract

The diversity of marine-derived Penicillium from Korea was investigated using morphological and multigene phylogenetic approaches, analyzing sequences of the internal transcribed spacer region, β-tubulin gene, and RNA polymerase subunit II gene. In addition, the biological activity of all isolated strains was evaluated. We tested for the extracellular enzyme activity of alginase, endoglucanase, and β-glucosidase, and antifungal activity against two plant pathogens (Colletotrichum acutatum and Fusarium oxysporum). A total of 184 strains of 36 Penicilliumspecies were isolated, with 27 species being identified. The most common species werePenicillium polonicum (19.6 %), P. rubens (11.4 %), P. chrysogenum (11.4 %), and P. crustosum(10.9 %). The diversity of Penicillium strains isolated from soil (foreshore soil and sand) and marine macroorganisms was higher than the diversity of strains isolated from seawater. While many of the isolated strains showed alginase and β-glucosidase activity, no endoglucanase activity was found. More than half the strains (50.5 %) showed antifungal activity against at least one of the plant pathogens tested. Compared with other strains in this study, P. citrinum(strain SFC20140101-M662) showed high antifungal activity against both plant pathogens. The results reported here expand our knowledge of marine-derived Penicillium diversity. The relatively high proportion of strains that showed antifungal and enzyme activity demonstrates that marine-derived Penicillium have great potential to be used in the production of natural bioactive products for pharmaceutical and/or industrial use.

DOI

10.1007/s10482-014-0205-5

Print ISSN

00036072

E-ISSN

15729699

Publisher Statement

Copyright © Springer International Publishing Switzerland 2014. Access to external full text or publisher's version may require subscription.

Full-text Version

Publisher’s Version

Recommended Citation

Park, M. S., Fong, J. J., Oh, S.-Y., Kwon, K. K., Sohn, J. ., & Lim, Y. W. (2014). Marine-derived Penicillium in Korea: Diversity, enzyme activity, and antifungal properties. Antonie van Leeuwenhoek, 106(2), 331-345. doi: 10.1007/s10482-014-0205-5