Title

Distinctive feature of microbial communities and bacterial functional profiles in Tricholoma matsutake dominant soil

Document Type

Journal article

Source Publication

PLoS ONE

Publication Date

12-15-2016

Volume

11

Issue

12

First Page

e0168573

Publisher

Public Library of Science

Abstract

Tricholoma matsutake, the pine mushroom, is a valuable forest product with high economic value in Asia, and plays an important ecological role as an ectomycorrhizal fungus. Around the host tree, T. matsutake hyphae generate a distinctive soil aggregating environment called a fairy ring, where fruiting bodies form. Because T. matsutake hyphae dominate the soil near the fairy ring, this species has the potential to influence the microbial community. To explore the influence of T. matsutake on the microbial communities, we compared the microbial community and predicted bacterial function between two different soil types—T. matsutake dominant and T. matsutake minor. DNA sequence analyses showed that fungal and bacterial diversity were lower in the T. matsutake dominant soil compared to T. matsu- take minor soil. Some microbial taxa were significantly more common in the T. matsutake dominant soil across geographic locations, many of which were previously identified as mycophillic or mycorrhiza helper bacteria. Between the two soil types, the predicted bacte- rial functional profiles (using PICRUSt) had significantly distinct KEGG modules. Modules for amino acid uptake, carbohydrate metabolism, and the type III secretion system were higher in the T. matsutake dominant soil than in the T. matsutake minor soil. Overall, similar microbial diversity, community structure, and bacterial functional profiles of the T. matsutake dominant soil across geographic locations suggest that T. matsutake may generate a domi- nance effect.

DOI

10.1371/journal.pone.0168573

E-ISSN

19326203

Funding Information

This study was supported by the National Institute of Forest Science (FP 0801-2009-01). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Statement

Copyright © 2016 Oh et al. Access to external full text or publisher's version may require subscription.

Additional Information

All relevant data except for sequence data are within the paper and its Supporting Information files. Sequence data are deposited in NCBI Sequence Read Archive (SRP046049).

Full-text Version

Publisher’s Version

Recommended Citation

Oh, S.-Y., Fong, J. J., Park, M. S. & Lim, Y. W. (2016). Distinctive feature of microbial communities and bacterial functional profiles in Tricholoma matsutake dominant soil. PLoS ONE, 11(12), 1-18. doi: 10.1371/journal.pone.0168573