Title

Diversity and saline resistance of endophytic fungi associated with pinus thunbergii in coastal shelterbelts of Korea

Document Type

Journal article

Source Publication

Journal of Microbiology and Biotechnology

Publication Date

3-28-2014

Volume

24

Issue

3

First Page

324

Last Page

333

Publisher

Han'gug Mi'saengmul Saengmyeong Gong Haghoe,Korean Society for Microbiology and Biotechnology

Keywords

Coastal pine forest, endophyte community, salinity tolerance, shelterbelt

Abstract

The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.

DOI

10.4014/jmb.1310.10041

Print ISSN

10177825

E-ISSN

17388872

Publisher Statement

Copyright © 2014 by The Korean Society for Microbiology and Biotechnology. Access to external full text or publisher's version may require subscription.

Full-text Version

Publisher’s Version

Recommended Citation

Min, Y. J., Park, M. S., Fong, J. J., Quan, Y., Jung, S., & Lim, Y. W. (2014). Diversity and saline resistance of endophytic fungi associated with pinus thunbergii in coastal shelterbelts of Korea. Journal of Microbiology and Biotechnology, 24(3), 324–333. doi: 10.4014/jmb.1310.10041