Sequence validation for the identification of the white-rot fungi Bjerkandera in public sequence databases
Journal of Microbiology and Biotechnology
Han'gug Mi'saengmul Saengmyeong Gong Haghoe,Korean Society for Microbiology and Biotechnology
Sequence validation, ITS, 28S nuclear ribosomal large subunit, white-rot fungi, Bjerkandera, GenBank
White-rot fungi of the genus Bjerkandera are cosmopolitan and have shown potential for industrial application and bioremediation. When distinguishing morphological characters are no longer present (e.g., cultures or dried specimen fragments), characterizing true sequences of Bjerkandera is crucial for accurate identification and application of the species. To build a framework for molecular identification of Bjerkandera, we carefully identified specimens of B. adusta and B. fumosa from Korea based on morphological characters, followed by sequencing the internal transcribed spacer region and 28S nuclear ribosomal large subunit. The phylogenetic analysis of Korean Bjerkandera specimens showed clear genetic differentiation between the two species. Using this phylogeny as a framework, we examined the identification accuracy of sequences available in GenBank. Analyses revealed that many Bjerkandera sequences in the database are either misidentified or unidentified. This study provides robust reference sequences for sequence-based identification of Bjerkandera, and further demonstrates the presence and dangers of incorrect sequences in GenBank.
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Jung. P. E., Fong, J. J., Park, M. S., Oh, S.-Y., Kim, C., & Lim, Y. W. (2014). Sequence validation for the identification of the white-rot fungi Bjerkandera in public sequence databases. Journal of Microbiology and Biotechnology, 24(10), 1313-1319. doi: 10.4014/jmb.1404.04021